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20X SSC – Autoclaved Sterile

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Molecular Biology hybridisation transfer buffer  saline-sodium citrate (SSC) buffer is used to control stringency for washing steps in protocols for  Southern Blotting, Northern Blotting, in situ hybridisation DNA microarrays. 20X SSC may be used to prevent drying of Agarose and PAGE gels during a vacuum transfer.

A 20X stock solution consists (Sterile Solution)

3M Sodium Chloride

300mM Tris-Sodium Citrate


DNase/RNase non detected.

0.2 micron filtered

Autoclaved Sterile


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