Laemmli Buffer 2X
Laemmli Buffer Solution 2X
Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min.
The 2-mercaptoethanol reduces the disulfide bonds and the SDS detergent denatures the proteins giving each an overall negative charge so that each will separate based on a poly- acrylamide gel by size.
The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to indicate the sample during loading. Glycerol increases the density of the sample so that it will layer in the sample well. Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.
pH: 6.8. Store at -20° C