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Biological Buffers Information

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Severn Biotech provides a range of pre-filtered formulated buffer concentrates for a range of Molecular and Cell Biology applications.  These products not only offer the busy lab considerable time and money saving but also eliminate errors in weighing, mixing and pH adjustment.

  • Made from ultra-pure reagents, including Molecular Biology Grade water (18 mega-ohm)
  • Filtered through 0.2 micron glass fibre and nylon to elimate particulate matter and bacteria
  • Each lot tested for DNase, RNase and protease activity
  • Water is LAL tested for pyrogens prior to use. (Limulus Amoebocyte Lysate Assay)

Typical Applications of Buffers: Genomic and Proteomic Studies

Gel Electrophoresis – TBE, TAE, TPE, Tris EDTA, Tris-Glycine
Western Blotting – Tris-Glycine, Maleic acid/Sodium Chloride buffer
Nucleic Acid Hybridisation – SSPE, SSC
Protein Denaturation – Tris-Glycine, SDS
RNA Electrophoresis –  MOPS EDTA
General Lab/Cell Culture – PBS, TBS, HEPES, MOPS EDTA, Maleic Acid

The following buffers are used in Cell Biology work as well as being useful in various Molecular Biology applications including electrophoresis:

  • MOPS (3-[morpholino]propanesulfonic acid) EDTA
  • HEPES (N-[2-hydroxyethyl]piperazine-N’-[2-ethanesulfonic acid])
  • TBS (tris buffered saline)
  • PBS (phosphate buffered saline)

PLEASE NOTE THIS IS NOT A PRODUCT PLEASE DO NOT ADD IT TO YOUR CART – THANK YOU