Saline-Sodium Citrate, SSC, 20X Solution-STERILE

Description:

Molecular Biology laboratory hybridisation transfer buffer saline-sodium citrate, SSC buffer.

Used to control stringency for washing steps in protocols for Southern Blotting, Northern Blotting, in situ hybridisation DNA microarrays.

20X SSC may be used to prevent drying of Agarose and PAGE gels during a vacuum transfer.

A 20X Sterile stock solution consists of:

3M Sodium Chloride

300mM Tris-Sodium Citrate

pH7.0

DNase/RNase non detected.

0.2 Micron filtered

Autoclaved Sterile