* Water Saturated Phenol Info
DNA and RNA are extracted using buffered redistilled water saturated phenol. The extraction of RNA is performed using phenol with a lower pH of 4.5.
DNA is extracted at a higher pH of 6-8.
Water Saturated Phenol is thus supplied as pH 4.5 and pH 6 and with a saturated Tris buffer solution to adjust the pH, supplied separately.
Tris buffered Pheol pH 8 is also manufactured as a ready to use solution and buffered to pH 8. Buffered water saturated phenol has a limited shelf life unless it is stored frozen or has 8-Hydroxyquinoline added.
Phenol Solution pH 8 is available with 8 -Hydroxyquinoline, anti-oxidant included.
Phenol/Chloroform/Iso-amyl alcohol is also supplied to enable the partitioning of DNA and RNA precipitating proteins from solution. The protein precipitates at the inter phase. At pH 7 both DNA and RNA partition in the upper aqueous phase. Below pH 7 DNA partitions with the protein fraction.
Phenol products are supplied in amber glass in order to reduce photo-oxidation. They are normally stored in a refrigerator at 4°C.
It is suggested that long term storage of Phenol solution pH 8 is carried out at minus 26°C in a freezer. Caution should be taken when freezing and defrosting to avoid bottle breakage. Slowly bring the solution to temperature by placing in a fridge to defrost, always do this with the bottle in its sealed bag provided.
PLEASE NOTE THIS IS NOT A PRODUCT PLEASE DO NOT ADD IT TO YOUR CART – THANK YOU