Saline-Sodium Citrate, SSC, 20X Solution

Description:

Molecular Biology hybridisation transfer buffer.

0.2 Micron filtered.

Saline-sodium citrate (SSC) buffer is used to control stringency for washing steps in protocols for Southern Blotting, Northern Blotting, in situ hybridisation DNA microarrays.

20X SSC may be used to prevent drying of Agarose and PAGE gels during a vacuum transfer.

A 20X stock solution consists of:

3M Sodium Chloride

300mM Tris-Sodium Citrate

pH7.0

DNase/RNase non detected.